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Scientific and Technical Bulletin of the Institute of Oilseed Crops NAAS (ISSN: 2078-7316) / 2025 / 39 / P. 56-63

Effect of various cytokinins on callus development and the frequency of shoot regeneration in flax callus culture
Soroka A. I.

This research explores the role of cytokinins — specifically 6-benzylaminopurine (BAP), thidiazuron (TDZ), and zeatin (ZEA)—in promoting flax callus development and shoot regeneration, using two distinct linseed genotypes. The main objective is to determine how different cytokinins influence callus growth and regeneration rates in flax, aiming to identify optimal conditions for enhanced shoot formation. Calli derived from hypocotyls of the linseed varieties Zaporozhsky Bogatyr and Evrika were cultivated on N6 media supplemented with BAP, TDZ, or ZEA at a concentration of 1 mg/L. Growth characteristics and shoot regeneration rates were assessed after four weeks. The study found that TDZ positively influenced callus development, while zeatin notably boosted the rate of shoot regeneration, particularly in the Evrika variety, which showed a threefold increase in shoot formation frequency. Overall, TDZ enhanced callus growth, and zeatin significantly improved shoot regeneration, highlighting the importance of cytokinin type and genotype in effective flax regeneration strategies.

Citation: Soroka, A. I. (2025). Effect of various cytokinins on callus development and the frequency of shoot regeneration in flax callus culture. Scientific and Technical Bulletin of the Institute of Oilseed Crops NAAS, 39, 56-63.

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